中药五白散对308nm准分子激光诱导色素沉着动物模型的影响(1)
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摘 要：目的：探讨中药五白散对308nm准分子激光诱导的色素沉着豚鼠模型的影响。方法：308nm准分子激光诱导色素沉着豚鼠模型。造模成功后，色素沉着区分为单纯基质乳膏组、2%氢醌组和中药五白散组，分别对应外涂于皮损区。外用治疗30天后，对各组的黑色素指数、黑素细胞、黑素颗粒及皮肤组织病理学检查观察药物的疗效和安全性。结果：五白散可降低黑色素指数水平，抑制黑素细胞活性，减少黑素生成，减轻UVB所致皮肤色素沉着的程度。结论：中药五白散对308nm准分子激光诱导豚鼠色素沉着有明显的改善作用，其机制可能是一定程度上抑制酪氨酸酶活性，减少黑素生成。
关键词：色素沉着；308nm准分子激光；中药；动物模型
中图分类号：R285.5
文献标识码：A
文章编号：1673-7717(2012)04-0893-03
Influence of Wubai Powder on Pigmentation Animal Model induced by 308nm Excimer Laser
ZHA Wei-feng1,SONG Wei-min2,AI Jun-jun3,ZHENG Jun-hui2,HU Ling-ling2
(1.Zhejiang Chinese Medical University，Hangzhou 310053，Zhejiang，China；
2.Hangzhou No.3 People's Hospital，Hangzhou 310009，Zhejiang，China；
3. Hangzhou Clinical College Affiliated to Anhui Medical University，Hangzhou 310009，Zhejiang，China)
Abstract:Objective：To observe the influence of Wubai Powder on pigmentation animal model induced by 308nm excimer laser. Methods：308nm excimer laser induced pigmentation animal model. After establishing the model，the areas of pigmentation were divided into 3 group：single matrix cream group，2% hydroquinone group and Wubai Powder group，and daubed them on corresponding lesions. After treamting for 30 days，the efficacy of the tested drug was evaluated comprehensively by measurements of melanin index，melanocyte，melanin as well as the histopathology of skin.Result：It was shown that the tested drug can decrease the level of melanin index，restrain the activity of melanocyte，reduce the generation of melanin and relieve pigmentation caused by UVB.Conclusion：Wubai Powder had obvious treatment function on 308nm excimer laser-induced pigmentation，its mechanism was to inhibit the activity of tyrosinase，which decreased melanin generation.
Key words:pigmentation；308nm excimer laser；traditional Chinese herb；animal model
近年来中药对皮肤色素沉着的改善作用被广泛关注，临床上外用中药美白制剂治疗黄褐斑等皮肤色素沉着有效。本研究选择对酪氨酸酶活性有显著抑制作用的5味中药，制成外用乳剂五白散，应用于308nm准分子激光诱导的皮肤色素沉着动物模型，通过观察黑色素指数、黑素细胞及黑素颗粒变化，可确定本实验模型是否建立成功，并通过以上各观察指标客观评价药物作用。
1 材料与方法
1.1 材料
1.1.1 实验动物采用普通级健康雄性棕黄色豚鼠20只，8周龄，体质量220～240g，由浙江中医药大学实验动物中心提供，委托该中心饲养。每只实验动物背部取3块相离区域，308nm准分子激光造模后外涂药物。
1.1.2 实验药物中药五白散由白芨、白芷、白蔹、白附子和白僵蚕组成，经杭州市第三人民医院制剂室制成乳膏制剂；2%氢醌乳膏购自广东人人康药业有限公司生产 ......
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